Coding

Part:BBa_K2012028

Designed by: Boyao Zhang   Group: iGEM16_HZAU-China   (2016-10-07)


PleD-His(6)Tag

PleD-His(6)Tag is the fusion protein of PleD(BBa_K2012002) and His-tag(BBa_J18909). PleD is a response regulator with a diguanylate cyclase (DGC) domain,an enzyme that turns GTP into c-di-GMP. With the fusion of his-tag,PleD protein can be easily detected and purified.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 14
    Illegal BamHI site found at 1172
    Illegal XhoI site found at 1366
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 430
    Illegal NgoMIV site found at 577
    Illegal AgeI site found at 913
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 87


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Categories
Parameters
None